Berberine Hydrochloride on Ultisil XB-C18

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Mixed Reference

ColumnUltisil XB-C18, 5μm, 4.6×250mm (P/N 00201-31043)
Mobile Phase0.01mol/L NH4H2PO4(adjust pH to 2.8 with phosphoric acid) : acetonitrile = 75:25
Detection345nm
Temperature20℃
Flow Rate1.0ml/min
Injection Volume10ul
NoteColumn temperature has great influence on separation.

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