Benzyl Isoquinolate on Ultisil AQ-C18
Impurity Reference Substance
| Column | Ultisil® AQ-C18 4.6×150 mm, 5 μm P/N: 00207-31041 |
| Detection | 220 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 µl |
Mobile Phase
A: 2.0061g Sodium perchlorate monohydrate, dissolve in 800mL water, add 0.5g triethylamine, adjust pH to 3.6 with phosphoric acid, add 200mL acetonitrile. B: 2.0049g Sodium perchlorate monohydrate, dissolve in 300mL water, add 0.5g triethylamine, adjust pH to 2.6 with phosphoric acid, add 700mL acetonitrile.
| Time | A% | B% |
| 0 | 100 | 0 |
| 5 | 100 | 0 |
| 45 | 0 | 100 |
| 60 | 0 | 100 |
| 61 | 100 | 0 |
| 67 | 100 | 0 |