Benzyl Isoquinolate on Ultisil AQ-C18

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Impurity Reference Substance

ColumnUltisil® AQ-C18 4.6×150 mm, 5 μm    P/N: 00207-31041
Detection220 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume10 µl

Mobile Phase

A: 2.0061g Sodium perchlorate monohydrate, dissolve in 800mL water, add 0.5g triethylamine, adjust pH to 3.6 with phosphoric acid, add 200mL acetonitrile. B: 2.0049g Sodium perchlorate monohydrate, dissolve in 300mL water, add 0.5g triethylamine, adjust pH to 2.6 with phosphoric acid, add 700mL acetonitrile.

TimeA%B%
01000
51000
450100
600100
611000
671000

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