Related substances Determination blank solvent

Gradient Program

Time(min)Mobile phase A(%)Mobile phase B(%)
07525
35955
64955
657525
717525
ColumnUltisil XB-C18 4.6×250 mm, 5 μm,
Mobile Phase of related substances DeterminationMobile phase A: Phosphate buffer (0.05mol/L dipotassium hydrogen phosphate solution, adjust pH to 8.2 with 20% phosphoric acid solution): acetonitrile=45:55
Mobile phase B: Methanol
Mobile Phase of Content DeterminationPhosphate buffer (0.05mol/L dipotassium hydrogen phosphate solution, adjust pH to 8.2 with 20% phosphoric acid solution): acetonitrile=45:55
Detection210 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume50 µl

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