Related Substance System Suitability
| Column | Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043 |
| Detection | 210 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.1 ml/min |
| Injection Volume | 50 µl |
Mobile Phase
A: 0.05mol/L dipotassium phosphate solution (Adjust pH to 8.2 with 20% phosphoric acid solution): Acetonitrile=45:55 B: Methanol
| Time | A% | B% |
| 0 | 75 | 25 |
| 35 | 95 | 5 |
| 64 | 95 | 5 |
| 65 | 75 | 25 |
| 71 | 75 | 25 |
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