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Azithromycin on Ultisil AQ-C18

Related Substance System Suitability

Column Ultisil® AQ-C18 4.6×250 mm, 5 μm P/N: 00207-31043
Detection 210 nm
Temperature 30 ℃
Flow Rate 1.1 ml/min
Injection Volume 50 µl

Mobile Phase

A: 0.05mol/L dipotassium phosphate solution (Adjust pH to 8.2 with 20% phosphoric acid solution): Acetonitrile=45:55 B: Methanol

Time A% B%
0 75 25
35 95 5
64 95 5
65 75 25
71 75 25