Column Ultisil® ODS-3（4.6×150mm，3.5μm) Mobile Phase Mobile phase A: Take 3.9g of sodium dihydrogen phosphate dihydrate, add it to 1000mL of water, mix well, and adjust the pH to 2.5 with phosphoric acid; take 950mL of the above solution, mix it with 50mL of acetonitrile and shake well, filter it with Read more…
Column Ultisil® SEC-120(7.8×300 mm,5 μm) Mobile Phase Pure Water Detection RID Temperature 35℃ Flow Rate 0.8 mL/min Injection Volume 20 μL If you have any problem or require further information, please contact info@welchmat.com
In HPLC, a solvent is the solution used to prepare the mobile phase, dissolve and transport the sample. The mobile phase is the main factor affecting the performance of HPLC, and its properties must meet the strict requirements of HPLC testing. 01UV detector Under the detection wavelength of the sample, Read more…
Column Ultisil® Diol (4.6×250 mm,5 μm) Mobile Phase n-hexane:isopropanol:10% glacial acetic acid=240:300:30 (add triethylamine to every 100ml mobile phase 0.05 mL), mixed with glacial acetic acid to adjust the pH to 5. Detection ELSD drift tube temperature: 90°C air flow: 3.5 bar Temperature 40℃ Flow Rate 1.0 ml/min InjectionVolume 20 Read more…
Column Ultisil® C18（4.6×250mm，5μm） Mobile Phase Methanol/water=98/2 Detection 280nm Temperature 25℃ Flow Rate 1.0 ml/min Injection Volume 20 μL Note It is recommended that the sample tray be controlled at 4°C If you have any problem or require further information, please contact info@welchmat.com
Column Ultisil® Cellu-J (4.6×250 mm,5 μm) Mobile Phase N-heptane: ethanol: diethylamine=98:2:0.1 Detection 240nm Temperature 25℃ Flow Rate 1.0 ml/min Injection Volume 10 μL
Column Ultisil® XB-C18（4.6×250mm，5μm) Mobile Phase Mobile phase A: 1.01g/L sodium heptanesulfonate solution (adjust pH to 2.6 with phosphoric acid) Detection 230nm Temperature 35℃ Flow Rate 1.0 ml/min InjectionVolume 10 μL If you have any problem or require further information, please contact info@welchmat.com
Number of theoretical plates (USP): (N, one of the column efficiency parameters of chromatography (referred to as column efficiency). N depends on the type and nature (particle size, particle size distribution, etc.), filling conditions, column length of the stationary phase, type and flow rate of the mobile phase, and the Read more…
I believe that most laboratory workers have more or less encountered an experimental phenomenon that makes everyone more headaches in daily testing: when we continuously inject samples, the retention time of the sample cannot be reproduced. For retention time skew and retention time fluctuation. Generally, when looking for the cause, Read more…
Using ultra-high-purity silica gel matrix,High-quality chromatographic packing materials produced by unique patented technology Excellent silica gel matrix ensures high mechanical strength of chromatographic packing materials Packing materials will bear high mechanical pressure during filling and separation and purification, so the mechanical strength requirements for packing are usually higher than those Read more…