Reference ascorbic acid, Ultisil® AQ-C18（4 .6×150mm，5 μm)
| Column | Ultisil® AQ-C18 (4.6×150mm，5μm) and Ultisil® ODS-3 (4.6×150mm, 5μm) |
| Wavelength | 270 nm |
| Temperature | 30℃ |
| Flow Rate | 0.8 mL/min |
| Injection Volume | 10 μL |
Mobile Phase
A%: 0.05mol/L potassium dihydrogen phosphate solution (adjust pH to 6.0 with triethylamine)
B%: acetonitrile
| Time | A% | B% |
| 0 | 100 | 0 |
| 5 | 100 | 0 |
| 34 | 80 | 20 |
| 39 | 30 | 70 |
| 42 | 30 | 70 |
| 47 | 100 | 0 |
| 55 | 100 | 0 |
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