Reference ascorbic acid, Ultisil® AQ-C18(4 .6×150mm,5 μm)
Column | Ultisil® AQ-C18 (4.6×150mm,5μm) and Ultisil® ODS-3 (4.6×150mm, 5μm) |
Wavelength | 270 nm |
Temperature | 30℃ |
Flow Rate | 0.8 mL/min |
Injection Volume | 10 μL |
Mobile Phase
A%: 0.05mol/L potassium dihydrogen phosphate solution (adjust pH to 6.0 with triethylamine)
B%: acetonitrile
Time | A% | B% |
0 | 100 | 0 |
5 | 100 | 0 |
34 | 80 | 20 |
39 | 30 | 70 |
42 | 30 | 70 |
47 | 100 | 0 |
55 | 100 | 0 |
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