Ascorbic Acid on Ultisil AQ-C18 and Ultisil ODS-3

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Reference ascorbic acid, Ultisil® AQ-C184 .6×150mm5 μm)

ColumnUltisil® AQ-C18 (4.6×150mm,5μm) and Ultisil® ODS-3 (4.6×150mm, 5μm)
Wavelength270 nm
Temperature30℃
Flow Rate0.8 mL/min
Injection Volume10 μL

Mobile Phase

A%: 0.05mol/L potassium dihydrogen phosphate solution (adjust pH to 6.0 with triethylamine)

B%: acetonitrile

TimeA%B%
01000
51000
348020
393070
423070
471000
551000

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