Mobile Phase 1: 0.4% triethylamine (pH 6.8 adjust with phosphoric acid)/methanol =10/90
| Column | Xtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043) |
| Mobile Phase | Mobile Phase 1: 0.4% triethylamine (pH 6.8 adjust with phosphoric acid)/methanol =10/90 Mobile Phase 2: 50 mmol/L sodium dihydrogen phosphate buffer (pH 4.5)/acetonitrile =60/40 |
| Detection | 254nm(Mobile Phase 1)； 220nm(Mobile Phase 2) |
| Temperature | 25℃(Mobile Phase 1)； 35℃(Mobile Phase 2) |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 20 µL |
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