Test solution(dissolve with ultrapure water)

ColumnWelch Ultisil® HILIC Amide (4.6×250mm,5μm)
Mobile PhaseAcetonitrile /0.05M potassium dihydrogen phosphate solution (pH =8.0) =50/50 (single pump)
Wavelength206 nm
Temperature30℃
Flow Rate1 mL/min
Injection Volume20 μl

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