Application of WelView Photochemical Reactor
Sulfonamides are derivatives of p-Aminobenzenesulfonamide, which are commonly used in aquaculture. With the exposure of the side effects of some sulfonamides and the improvement of people’s safety awareness, the residue of sulfonamides in animal foods is strictly restricted now.
Animal derived food
Methods for the detection and derivation of sulfonamides
Methods for the detection of sulfonamides residues are multiple, including HPLC, enzyme-linked immunoassay, Charm Ⅱ radioimmunoassay, GC, thin-layer chromatography and so on. One of the most commonly used methods is HPLC-fluorescence detector method. Some sulfonamides do not have fluorescence, but they can be detected by fluorescence detector after being derived.
In the detection of sulfonamides, post-column chemical derivation and post-column photochemical derivation are mainly used. Photochemical derivation is more popular because it does not require any chemical derivative reagents and reagent pumps. It also reduces the cleaning of liquid phase system and extends the lifetime of the instrument.
The following is a comparison of the results of the detection of six sulfonamides by fluorescence detectors and photochemical derivatization.
Chromatograph: Welch Wisys 5000 HPLC
Column: Ultisil® XB-C18, 5μm, 4.6×250 mm
Derivator: WelView photochemical reactor
Flow rate : 1.0 mL/min
Fluorescence detector: excitation wavelength 232 nm, emission wavelength 400 nm
Injection volume :20μL
Gradient elution table:
WelView Photochemical Reactor
|Display screen||2.19 inch colour screen|
|Maximum withstand voltage||3000psi (207bar)|
|Maximum flow rate||3ml/min|
|Leak fluid alarm||Leakage alarm|
|Life of lamp||About 9000 hours|
|Time spent by UV lamp||UV lamps use real – time timing, color screen display|
|Constant temperature method||Air patenting|
|Weight kg||Almost 3.0kg|
|Ambient temperature: ℃||0~45|
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