Sulfonamides are derivatives of p-Aminobenzenesulfonamide, which are commonly used in aquaculture. With the exposure of the side effects of some sulfonamides and the improvement of people’s safety awareness, the residue of sulfonamides in animal foods is strictly restricted now.

Animal derived food

Methods for the detection and derivation of sulfonamides

Methods for the detection of sulfonamides residues are multiple, including HPLC, enzyme-linked immunoassay, Charm Ⅱ radioimmunoassay, GC, thin-layer chromatography and so on. One of the most commonly used methods is HPLC-fluorescence detector method. Some sulfonamides do not have fluorescence, but they can be detected by fluorescence detector after being derived.

Photochemical derivation after column HPLC configuration

Chemical derivation after column HPLC configuration

In the detection of sulfonamides, post-column chemical derivation and post-column photochemical derivation are mainly used. Photochemical derivation is more popular because it does not require any chemical derivative reagents and reagent pumps. It also reduces the cleaning of liquid phase system and extends the lifetime of the instrument.

The following is a comparison of the results of the detection of six sulfonamides by fluorescence detectors and photochemical derivatization.


 Chromatographic conditions

 Chromatograph: Welch Wisys 5000 HPLC

 Column: Ultisil® XB-C18, 5μm, 4.6×250 mm

 Derivator:  WelView photochemical reactor

 Flow rate : 1.0 mL/min

 Fluorescence detector: excitation wavelength 232 nm, emission wavelength 400 nm

 Injection volume :20μL

Gradient elution table:


WelView Photochemical Reactor

Display screen 2.19 inch colour screen
Maximum withstand voltage3000psi (207bar)
Maximum flow rate3ml/min
Leak fluid alarmLeakage alarm
Life of lampAbout 9000 hours
Time spent by UV lampUV lamps use real – time timing, color screen display
Anti-interferenceAnti-electric interference
Constant temperature methodAir patenting
Electric source220V/50Hz
Power W22
Weight kgAlmost 3.0kg
Ambient temperature: ℃0~45

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