Chiral chromatography columns are commonly used for the separation of chiral isomers. In addition to chiral isomers, they also demonstrate certain effectiveness in separating other structural isomers or components with closely related structures.
Application Example:
Separation of Cyanobenzyl Chloride & m-Cyanobenzyl Chloride
The structures are shown in the diagram:
In the case of these two substances, which are meta and para isomers, various reversed-phase chromatography columns with different selectivities were attempted, but no separation trend was observed, indicating the inability to separate them. Finally, separation was achieved using a chiral chromatography column.
Chromatographic Conditions:
Column: Ultimate® Cellu-D 4.6*250mm, 5μm.
Mobile Phase: Hexane/Isopropanol = 98/2.
0 Comments