Contrast solution
| No. | Rt (min) | Area | Height | Plates (USP) | Tailing Factor | Resolution (USP) |
| 1 | 13.482 | 1544587 | 128378 | 27820 | 0.987 | — |
| 2 | 50.375 | 926924 | 93853 | 561657 | 0.963 | 124.594 |
| Column | Welch Ultisil®LP-C18（4.6×250mm，5μm） |
| Detector | 236 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0ml/min |
| Injection Volume | 10ul |
Mobile phase A: acetonitrile; mobile phase B: 0.01% phosphoric acid solution
Gradient Program
| Time | Mobile Phase A（%） | Mobile Phase B（%） |
| 0 | 7 | 93 |
| 22 | 18 | 82 |
| 29 | 18 | 82 |
| 31 | 23 | 77 |
| 40 | 23 | 77 |
| 53 | 40 | 60 |
| 60 | 40 | 60 |
| 65 | 7 | 93 |
If you have any problem or require further information, please contact info@welchmat.com.
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