Sample
| Column | Ultisil® LP-C18, 5μm, 4.6×150mm PN: 00208-31041 |
| Detection | 280 nm |
| Temperature | Room temperature |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: 0.2% acetic acid Solution B: acetonitrile
| Time (min) | A (%) | B (%) |
| 0 | 90 | 10 |
| 50 | 50 | 50 |
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