Mixed Reference
| Column | Ultisil PFP, 4.6×250mm, 5μm (P/N 00224-31043) |
| Detection | 530nm |
| Temperature | 30℃ |
| Flow Rate | 0.8ml/min |
| Injection Volume | 10ul |
Mobile Phase
| Time/min | 5.0% formic acid | Methanol |
| 0 | 92 | 8 |
| 5 | 90 | 10 |
| 22 | 90 | 10 |
| 30 | 85 | 15 |
| 35 | 80 | 20 |
| 40 | 65 | 35 |
| 45 | 50 | 50 |
| 50 | 35 | 65 |
| 55 | 30 | 70 |
| 55.1 | 92 | 8 |
| 65 | 92 | 8 |
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