System adaptability test

ColumnWelch Ultisil XB-C18 4.6×50mm,1.8μm (P/N:00201-11037)
Mobile PhaseMethanol-sodium dihydrogen phosphate buffer salt (6g sodium dihydrogen phosphate solution + 1ml triethylamine mixed with water to 1000ml, adjust pH to 7.0 with sodium hydroxide solution ) = 28:72
Detector254nm
Temperature30℃
Flow Rate1ml/min
Injection Volume10ul
NotesThe concentration of sodium hydroxide solution is: 0.02M

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