Related Substance Sample Solution

ColumnUltisil® LP-AQ, 5μm, 4.6×250mm    PN: 00259-31043
Detection254 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl

Mobile Phase

The standard requires that the gradient run immediately after ampicillin peak elution. (Ampicillin peak was eluted in 10min)

TimeA%B%
05545
105050
405050
554852
604852
704060

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