Sensitive solution (ampicillin)
| Column | Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043 |
| Detection | 254 nm |
| Temperature | 35℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution – acetonitrile – water= 0.5:50:50:900 B: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution – acetonitrile – water= 0.5:50:40 0-6 00
| Time (min) | A (%) | B (%) |
| 0 | 85 | 15 |
| 10 | 85 | 15 |
| 20 | 0 | 100 |
| 110 | 0 | 100 |
| 115 | 85 | 15 |
| 125 | 85 | 15 |
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