Ampicillin and Probenecid Capsules related substance on Xtimate C18

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Sensitive solution (ampicillin)

ColumnXtimate C18, 5μm, 4.6×250mm    PN: 00101-21043
Detection254 nm
Temperature35℃
Flow Rate1 ml/min
Injection Volume20 µl

Mobile Phase

A: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution – acetonitrile – water= 0.5:50:50:900 B: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution – acetonitrile – water= 0.5:50:40 0-6 00

Time (min)A (%)B (%)
08515
108515
200100
1100100
1158515
1258515

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