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Ampicillin and Probenecid Capsules related substance on Xtimate C18

Sensitive solution (ampicillin)

Column Xtimate C18, 5μm, 4.6×250mm PN: 00101-21043
Detection 254 nm
Temperature 35℃
Flow Rate 1 ml/min
Injection Volume 20 µl

Mobile Phase

A: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution - acetonitrile - water= 0.5:50:50:900 B: 12% acetic acid solution -0.2mol/L dipotassium hydrogen phosphate solution - acetonitrile - water= 0.5:50:40 0-6 00

Time (min) A (%) B (%)
0 85 15
10 85 15
20 0 100
110 0 100
115 85 15
125 85 15