Solution of test sample
| # | RT | Area | Height | Area% | Plates | Tailing factor | Resolution |
Gradient Program
| Time（min） | A% | B% |
| 0 | 92 | 8 |
| 10 | 92 | 8 |
| 35 | 0 | 100 |
| 50 | 0 | 100 |
| 51 | 92 | 8 |
| 65 | 92 | 8 |
| Column | Welch Ultisil® Polar RP 4.6×250mm，5μm (P/N: 00215-31043) |
| Mobile Phase | A: (0.05 mol/L potassium dihydrogen phosphate solution, adjust pH to 5.0 with 2 mol/L potassium hydroxide solution): acetonitrile = 99:1 B：(0.05 mol/L potassium dihydrogen phosphate solution, adjust pH to 5.0 with 2 mol/L potassium hydroxide solution): acetonitrile = 80:20 |
| Detector | 254nm |
| Temperature | 30℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20ul |
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