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Amoxicillin and Clavulanate Potassium on Ultisil LP-C18

Sample

Column Ultisil® LP-C18, 5μm, 4.6×300mm PN: 00208-31044
Detection 254 nm
Temperature 25℃
Flow Rate 1 ml/min
Injection Volume 20 µl

Mobile Phase

A: 10mmol/L monopotassium phosphate(pH6.0) B: 10mmol/L monopotassium phosphate (pH6.0): acetonitrile=20:80

Time (min) A (%) B (%)
0 95 5
0.5 95 5
30.5 59 41
32 95 5
40 95 5