ColumnXtimate® C8, 4.6×250mm, 5μm (P/N: 00102-21043)
Mobile PhaseSodium heptane sulfonate solution (0.6g of sodium heptane sulfonate, dissolved in 600 ml water, adjust pH 2.4 with Sulfuric acid) /acetonitrile = 60/40
Detection230 nm
Temperature25 ℃
Flow Rate1.0 mL/min
Injection Volume20 µL

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