| Column | Ultisil® AQ-C18 4.6×150 mm, 3 μm P/N: 00207-21041 |
| Detection | 244 nm |
| Temperature | 20 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 10 µl |
Mobile Phase
A: 0.01mol/L Monopotassium Phosphate solution (Adjust pH to 5.0 with sodium hydroxide solution) B: Acetonitrile
| Time | A% | B% |
| 0 | 55 | 45 |
| 5 | 50 | 50 |
| 10 | 50 | 50 |
| 15 | 48 | 52 |
| 20 | 48 | 52 |
| 25 | 40 | 60 |
| 30 | 55 | 45 |
| 40 | 55 | 45 |
If you have any problem or require further information, please contact info@welchmat.com.
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