Assaying Reference Substance

ColumnUltisil® XB-C30 4.6×250 mm, 5 μm    P/N: 00223-31043
Detection474 nm
Temperature30 ℃
Flow Rate1.0 ml/min
Injection Volume20 µl
NotesSensitive to injection volume and dissolved solvent

Mobile Phase

A: Methanol-1% Phosphoric acid solution=94:4 B: Methanol- Tert-butyl methyl ether- 1% Phosphoric acid solution=16:80:4

TimeA%B%
06733
155248
230100
276733
306733

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