Looking back at the previous part of the article “All About Welch C18 Columns-Let’s have a closer look”, we have completely introduced several most common Welch C18 columns . This article mainly brings up 1.8 μm ultra high pressure analysis column and core-shell analysis column are introduced in detail. And analyzes the characteristics of UHPLC chromatographic column and ultimate core-shell liquid chromatographic column, and subdivides these two types of chromatographic columns into different types of chromatographic columns. It will be introduced from the pH range they can withstand and the characteristics of subdivided chromatographic columns.

Characteristics of UHPLC column

  1. Ultra resolution: achieve the same or even better resolution of the general chromatographic column when it is shorter, finer and less filler than the general chromatographic column.
  2. Ultra speed: UHPLC can provide more information per unit time on the premise of ensuring the same quality data.
  3. High sensitivity: improve the column efficiency N, so that the slit width W becomes narrower, but the peak height increases. At the same time, because UHPLC uses a shorter column (column length L is smaller), the peak height is further increased. Therefore, while improving the column efficiency, 1.8 μm The UHPLC system is better than 5 μm And 3.5 μm The sensitivity of the system is increased by 70% and 40% respectively, and the sensitivity can be increased by 3 times and 2 times respectively when the column efficiency is the same.

Xtimate ® UHPLC-C18
Tolerance pH range: 1.0-12.5

Characteristic

  • 1.8μm high purity silica gel, withstand pressure up to 15,000 psi;
  • Selectivity is the same as 5 μm Xtimate C18, because the same bonding process is used, the method can be easily transferred.

Ultisil ® UHPLC XB-C18
Tolerance pH range: 1.5-10.0

Characteristic

  • 1.8μm high purity silica gel, withstand pressure up to 15,000 psi;
  • Selectivity is the same as 5 μm XB-C18, because the same bonding process is used, the method can be easily transferred.

Ultisil ® UHPLC AQ-C18
Tolerance pH range: 1.5-10.0

Characteristic

  • 1.8μm high purity silica gel, withstand pressure up to 15,000 psi;
  • Selectivity is the same as 5 μm AQ-C18, because the same bonding process is used, the method can be easily transferred.

Ultisil ® UHPLC LP-C18
Tolerance pH range: 0.5-8.0

Characteristic

  • 1.8μm high purity silica gel, withstand pressure up to 15,000 psi;
  • Selectivity is the same as 5 μm LP-C18, because the same bonding process is used, the method can be easily transferred.

Ultisil ® UHPLC Polar RP
Tolerance pH range: 1.5-10.0

Characteristic

  • 1.8μm high purity silica gel, withstand pressure up to 15,000 psi;
  • The selectivity of Polar RP is the same as 5μm, because the same bonding process is used, the method can be easily transferred to universal C18.

Characteristics of Boltimate column

  1. Innovative particle technology.
  2. It has the advantages of ultra-high resolution, high separation and high column efficiency of the sub-2 μm column, while the column reverse pressure is less than 50% of the sub-2 μm column.
  3. Compared with the traditional 3μm and 5μm analytical columns, the efficiency, speed, separation and sensitivity of the column are greatly improved. Reduce diffusion path and improve column efficiency.
  4. Narrower particle distribution and 2μm sieve plate for the column avoid the disadvantage of sub-2 μm column which is very easy to increase the column pressure. It still has stable and reliable high performance and is more durable for complex matrix samples.
  5. Perfectly compatible with any existing HPLC/UHPLC liquid phase equipment.
  6. Supports high pressure conditions up to 600bar

Boltimate ® C18
Tolerance pH range: 2.0-8.5

Characteristics

  • Universal type C18;
  • For acid, alkali, neutral compounds have suitable retention capabilities
  • High-resolution.

Boltimate ® EXT-C18
Tolerance pH range: 1.5-12.0

Characteristics

  • The pH tolerance of silica gel was greatly enhanced by organic-inorganic hybridization of porous layer.
  • Universal type C18.

Boltimate ® LP-C18
Tolerance pH range: 0.5-8.0

  • Resistant to 100% pure water phase;
  • Side chain steric protection shields hydroxyl groups;
  • Non sealing process;
  • It has strong spatial position selectivity and strong orthogonality with conventional C18. Ideal baseline noise can be obtained by ELSD detector under strong acid conditions, indicating that the loss of bonding phase is small under strong acid conditions;
  • Strong acid, high aqueous phase, ELSD, MS are preferred.

If you have any problem or require further information, please contact info@welchmat.com.

Categories: News

0 Comments

Leave a Reply

Avatar placeholder

Your email address will not be published. Required fields are marked *