Albumin on Ultisil AQ-C18
Albumin Reference Substance
| Column | Ultisil® XB-C4 4.6×250 mm, 5 μm, 300Å P/N: 00216-33043 |
| Detection | 215 nm |
| Temperature | 30 ℃ |
| Flow Rate | 1.0 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: 0.1% TFA aqueous solution B: 0.1% Trifluoroacetic acid acetonitrile solution
| Time | A% | B% |
| 0 | 80 | 20 |
| 5 | 80 | 20 |
| 13 | 50 | 50 |
| 20 | 35 | 65 |
| 23 | 5 | 95 |
| 24 | 80 | 20 |
| 30 | 80 | 20 |