Test sample solution
Gradient Program
| Time（min） | A% | B% |
| 0 | 60 | 40 |
| 20 | 60 | 40 |
| 35 | 50 | 50 |
| 50 | 20 | 80 |
| 60 | 20 | 80 |
| 60.1 | 60 | 40 |
| 80 | 60 | 40 |
| Column | Welch Ultisil® XB-C18 4.6×250mm，3μm (P/N: 00201-21043) |
| Mobile Phase | A:10mmol/L ammonium acetate solution B:Acetonitrile / methanol = 50/30 |
| Detector | 259nm |
| Temperature | 50℃ |
| Flow Rate | 0.6ml/min |
| Injection Volume | 10ul |
| Notes | After using，the column should be cooled to room temperature and change mobile phase to wash it |
If you have any problem or require further information, please contact info@welchmat.com.
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