Adenine on Ultisil XB-C18

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ColumnUltisil XB-C18, 4.6×250mm, 5μm (P/N 00201-31043)
Mobile Phase0.63g L-phenylalanine, 0.47g bluestone, 2.21g ammonium dihydrogen phosphate, dissolve in acetonitrile(38ml)-water(962ml) solution
Detection274nm
Temperature25°C
Flow Rate0.6ml/min
Injection Volume20µl
Operation Time25min
S-isomer tR~0.9
NotesOver-night mobile phase equilibration at 0.2ml/min

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