Mixed reference standard
Column | Ultisil® XB-C18 4.6×250 mm, 5 μm |
Mobile Phase | Mobile phase A: methanol: water: acetic acid=50:950:1 Mobile phase B: methanol: water: acetic acid=500:500:1 |
Detection | 254 nm. 275 nm, 317 nm |
Temperature | 40°C |
Flow Rate | 0.9 ml/min |
Injection Volume | 20 µl |
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