9-(2-Phosphonylmethoxypropyl)-adenine on Xtimate C18

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Part of the Scale

Gradient Program

Time(min)AB
0955
305050
355050
35.01955
45955
ColumnXtimate® C18, 4.6×250 mm, 5 μm (P/N: 00101-21043)
Mobile PhaseMobile phase A: 0.1M ammonium acetate (adjust pH to 3.8 with acetic acid) Mobile Phase B: methanol/acetonitrile =70/30
Detection260 nm
Temperature30℃
Flow Rate1.0 mL/min
Injection Volume20 µL

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