Self reference substance
| Column | Welch UltisilTM XB-C18 4.6×150mm，5μm (P/N: 00201-31041) |
| Mobile Phase | Methanol-acetonitrile-buffer solution = 15:15:70 |
| Detector | 254nm |
| Temperature | 35℃ |
| Flow Rate | 1.2ml/min |
| Injection Volume | 20ul |
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