| Column | Welch Ultisil ® XB C18（2.1×50mm，1.8μm） |
| Column Temperature | 35 ℃ |
| Flow Rate | 0.3 ml/min |
| Injection Volume | 1 μL |
| Note | If there is still residual doxycycline after high-concentration injection, it is recommended to increase the elution time of EDTA-2Na. EDTA-2Na cannot be directly introduced into mass spectrometry, so it is necessary to switch to a bypass wash during the cleaning process. |
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