ColumnXtimate® C18, 4.6×250mm, 5μm (P/N: 00101-21043)
Mobile PhaseMethanol-0.05 mol/L potassium dihydrogen phosphate solution (add 2% triethylamine, adjust pH 4.75 with phosphoric acid) =10:90
Detection254 nm
Temperature30 ℃
Flow Rate1.0 mL/min
Injection Volume20 µL

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