Sample Solution

ColumnUltisil LP-C18, 5μm, 4.6×250mm (P/N 00208-31043)
Detection280nm
Temperature30℃
Flow Rate1.0ml/min
Injection Volume40ul

Mobile Phase

Time/min0.1% phosphoric acidMethanol
0-307030
30-3570-2030-80
35-452080

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