Solution of mixed standards
| Column | Welch Ultisil® LP-C18（4.6×300mm，5μm) |
| Wavelength | 280 nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 5 μL |
Mobile Phase
| Time | Methanol(%) | Water(%) |
| 0 | 75 | 25 |
| 12 | 75 | 25 |
| 15 | 100 | 0 |
| 22 | 100 | 0 |
| 23 | 75 | 25 |
| 25 | 75 | 25 |
| 30 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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