Test solution -20ul
| Column | Welch Ultisil XB-C18(4.6×150mm，5um) |
| Detector | 230nm |
| Temperature | 35℃ |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 μL |
Mobile Phase
| Time | Water(%) | Methanol(%) |
| 0 | 75 | 25 |
| 10 | 75 | 25 |
| 20 | 0 | 100 |
| 21 | 0 | 100 |
| 22 | 75 | 25 |
| 30 | 75 | 25 |
If you have any problem or require further information, please contact info@welchmat.com.
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