Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.
Features
- Ultra-pure spherical porous silica, purity > 99.999%
- High efficiency: theoretical plate > 80000/m
- Excellent peak symmetry: tailing factor=0.95~1.05
- Exceptional lot-to-lot reproducibility
SEM Pictures of Ultisil Particles
Trace Amount Metal Contents Test
A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Separation of Basic Compounds Antidepressant at pH 7.0
Capacity Factor(K’) of Batch to Batch Reproducibility
Brand: Ultisil
Phase: LP-C18
Particle Size: 3.5μm
Pore Size: 120Å
USP Classification: L1
Separation Mode: Reversed Mode
pH Range: 0.5-8.0
Surface Area: 320m2/g
Carbon Load: 10%
Max.Pressure: 60Mpa
Endcapped: No
Max.Temp.: 70°C(pH≤6.5) and 40°C(pH>6.5)
Particle Shape: Spherical
Ultisil Series HPLC Columns are based on ultra-pure ( purity > 99.999% ) spherical and totally porous silica, unique bonding chemistry and proprietary surface modification techniques, producing excellent peak shape, column efficiency and exceptional lot-to-lot reproducibility. Ultisil column is the best choice for method development, owing to complete bonding chemistries and stable performance.
Features
- Ultra-pure spherical porous silica, purity > 99.999%
- High efficiency: theoretical plate > 80000/m
- Excellent peak symmetry: tailing factor=0.95~1.05
- Exceptional lot-to-lot reproducibility
SEM Pictures of Ultisil Particles
Trace Amount Metal Contents Test
A useful chromatographic test of trace amount of metal contents in the column is to compare the peak symmetry of one pair of positional isomers, 4,4’-dipyridyl and 2,2’-dipyridyl, and a neutral chelating reagent, 1,2-dihydroxylnaphthalene. 4,4’-dipyridyl, which cannot form chelating complex with metal, is used as a reference. 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene, which are chelating reagents, are sensitive to trace amount metal in silica. When a C18 column based on type A silica or other so-called type B silica with higher metal content is used, the peaks of 2,2’-dipyridyl and 1,2-dihydroxylnaphthalene would tail or even totally disappear.
Separation of Basic Compounds Antidepressant at pH 7.0
Capacity Factor(K’) of Batch to Batch Reproducibility
Brand: Ultisil
Phase: LP-C18
Particle Size: 3.5μm
Pore Size: 120Å
USP Classification: L1
Separation Mode: Reversed Mode
pH Range: 0.5-8.0
Surface Area: 320m2/g
Carbon Load: 10%
Max.Pressure: 60Mpa
Endcapped: No
Max.Temp.: 70°C(pH≤6.5) and 40°C(pH>6.5)
Particle Shape: Spherical