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Separation of vitamin K2 and its isomers using Ultisil XB-C30

Test Report

Chromatography conditions:

Column

Ultisil ® XB-C30(4.6×250mm,5μm)

Mobile Phase

A: Acetonitrile/water=90/10

B: Acetonitrile

Detector

UV

Injection Volume

10 μL

Column temperature

10℃

Flow Rate

1.0 ml /min

Detection wavelength:

270 nm

Elution program

Time(min)

Mobile Phase A (%)

Mobile Phase B (%)

0

100

0

20

0

100

35

0

100

35.1

100

0

45

100

0

 

Note

/

Chromatogram and Data: 

  1. Mixed standard solution chromatogram

Conclusion:
Under these chromatographic conditions, Welch Ultisil® XB-C304.6×250mm5μmcan meet the detection requirements.

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