Determination of unknown sample content using Ultisil HILIC Amphion II
Test Report
Chromatography conditions:
Instrument Info |
Shimadzu LC-20AD |
Column |
Ultisil®HILIC Amphion II(4.6×250mm,5um) |
Mobile Phase |
Acetonitrile/5.5g/L ammonium acetate (use acetic acid to adjust pH to 6.0) =65/35 |
Injection Volume |
5 μL |
Flow Rate |
0.8 ml /min |
Detector conditions |
Excitation wavelength: 274nm; Emission wavelength: 312nm; |
Column Temperature |
50℃ |
Note |
/ |
Chromatogram and Data:
- Detection amplification chart of mixed standard solution
Retention time(min) |
Area |
Height |
Number of theoretical plates(USP) |
Tailing Factor |
Resolution(USP) |
8.809 |
8125562 |
684851 |
14662 |
1.234 |
-- |
2. Detection chart of test solution
Retention time(min) |
Area |
Height |
Number of theoretical plates(USP) |
Tailing Factor |
Resolution(USP) |
8.804 |
9418389 |
803126 |
14552 |
1.210 |
-- |
Conclusion:
Under these chromatographic conditions, Welch Ultisil®HILIC Amphion II(4.6×250mm,5um)can meet the detection requirements.