Determination of Calais by Ultisil LP-C8
Sample Solution 2 (0.4mg/ml solid 1 and 0.8ug/ml solid 2 mixed solution):
| Column | Ultisil® LP-C8, 5μm, 4.6×250mm PN: 00209-31043 |
| Detection | 285 nm |
| Temperature | 40℃ |
| Flow Rate | 1 ml/min |
| Injection Volume | 20 µl |
Mobile Phase
A: 1mL TFA add into 1000mL water B: Acetonitrile
| Time (min) | A% | B% |
| 0 | 85 | 15 |
| 3 | 85 | 15 |
| 30 | 5 | 95 |
| 33 | 5 | 95 |