Butenafine Hydrochloride on Welchrom C18
| Column | Welchrom® C18, 4.6×250mm, 5μm (P/N: 00310-02043) |
| Mobile Phase | Acetate buffer (18 g sodium acetate, 9.8 mL glacial acetic acid, diluted with water to 1000 mL)/methanol /isopropanol =17/70/3 |
| Detection | 282 nm |
| Temperature | Ambient |
| Flow Rate | 1.0 mL/min |
| Injection Volume | 10 µL |
| Notes | The project should be protected from light; 2. The buffer solution should be prepared again the next day. |